Optimization of culture conditions of Lactobacillus plantarum using cassava lees liquid

Increasing the viable bacteria counts of lactic acid bacteria in the medium and cutting down cost of medium production were the key of developing feeding lactic acid bacteria agent.The fermentation medium and fermentation parameters of Lactobacillus plantarum cultured in cassava lees liquid were optimized by single factor and orthogonal experiment method.The results showed that the optimum medium of Lactobacillus plantarum including cassava lees liquid 97.75%,molasses 2%,yeast extract 0.25%,pH5.5 and the optimum fermentation parameters were shake cultivation at 7℃,120 r/min for 24 h.In the end of culture,viable bacteria counts of Lactobacillus plantarum was up to 49.0 × 108 cfu/ml,which was 2.41 times of that before optimizing,and 35% higher than in the MRS medium.The results showed that it was feasible to culture Lactobacillus plantarum using cassava lees liquid.