Transfection by green fluorescent protein gene and cloned culture of cell line of hepatocellular carcinoma

Purpose:To study the establishment of stable,high level green fluorescent protein (GFP) expressing cell line of hepatocellular carcinoma(HCC) and pass on its culture contiuously.Methods:7721 cell line of HCC was transfected by retrovirus vector with integrated GFP cDNA. GFP expressing cancer cells were detected by fluorescent microscopy through G418 selecting and cloned culture.Results:Most of the cancer cells were dead 5 days after being transfected by GFP, scattered or clustered green fluorescence can be seen by microscopy. In culture for 65 days,almost all of the clone 28 cells expressed high intensity GFP fluorescence and stability.Conclusions:7721 GFP cell line can provide a basis for establishing an ideal animal model for research of tumor invasion and metastasis.