Research Development on Zinc Finger Nucleases and Its Application

Zinc finger nuclease was made of zinc finger and nonspecific cleavage nuclease.Zinc fingers could bind the double-stranded DNA specially.Non-specific cleavage nucleases could make double-stranded DNA break in chromosomal.The target gene mutation such as gene mutation or exogenous gene insertion happened by repairing mechanismin vivo.It could be used in transgentic animal production normally.The development of the research on zinc finger,cleavage enzyme and production of transgentic animal using zinc finger nucleases was summarized in this review.The discovery and the structure of zinc finger,its binding manners with DNA,selecting and designing methods were first discussed.Then the discovery of cleavage enzyme,charactersistics of FokⅠ on cutting the DNA double strains,and specialities of engineered cleavage enzyme for cutting DNA double strains after heterodimer forming were analysed.