Survivin antisense oligodeoxy-nucleotid and AsI_3-cys induce apoptosis of K562 cells

Objective To investigate the effect of survivin antisense oligodeoxy-nucleotid(ASODN) and AsI3-cys on cell proliferation and cell apoptosis of K562 cells. Methods K562 cells were serially subcultured. Survivin ASODN was transfected into K562 cells by lipofectamine mediated transient transfection method. Cell proliferation was measured by MTT method and cell apoptosis index was detected by Tunel,RT-PCR was used to detect survivin gene expression,FCM was used to detect apoptosis rate and cell cycle. Results Survivin ASODN had obvious inhibitory effects on the cell proliferation and induced cell apoptosis of K562 cells. The cell apoptosis rate in Survivin ASODN and AsI3-cys group was obviously higher than three control groups. Conclusion Survivin ASODN can induce cell apoptosis and enhance AsI3-cys induced apoptosis in K562 cells.