Genetic diversity of four populations of Calycanthus chinensis based on ISSR and RAPD markers

Genetic diversities of 4 populations 25 individuals of Calycanthus chinensis were studied by ISSR and RAPD marker. 62 loci were identified with 10 simple sequence repeat primers screened from 60 primers,out of which 41 loci were polymorphic and the percentage of polymorphic bands was 65.60%;52 loci were identified with 10 oligonucleotide primers screened from 60 primers,out of which 31 loci were polymorphic and the percentage of polymorphic bands was 57.50%. Shannon’s indices of diversity (I) were 0.3737 among the species level with the average of 0.1645 among populations. Nei’s gene diversity(h) was 0.2528 among the species with the average of 0.1117 among populations;and the effective number of alleles (ne) was 1.4473 among the species with the average of 1.1972 among populations. Genetic variance analysis showed the coefficient of gene differentiation (Gst) was 0.5297 at species level,and the gene flow (Nm) was 0.4450. Cluster analysis indicated that 4 populations could be divided into 2 groups and consistent with their geographical distribution. As a highly restricted geographical distribution species,the genetic diversity level of C.chinensis was not high. It is necessary to take measures to protect in situ or ex situ endangered species.