2006•Journal of Practical Diagnosis and TherapyRequires access
Relationship between apoptosis in HT-29 cell induced by ursolic acid and expression of caspase-9,bcl-2 and bax
Tan Ji
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Abstract
Objective To study the antitumor effect of ursolic acid and its mechanism.Methods The human colorectal carcinoma HT-29 cells were treated with ursolic acid.The proliferation inhibitory was examined by MTT method.Morphological study,TUNEL method and flow cytometry were used to detect apoptosis.Immunohistochemical method was used to detect the expression of apoptosis related gene caspase-9,bcl-2 and bax.Results Ursolic acid inhibited the proliferation of HT-29 cell.HT-29 cell treated with ursolic acid was induced to apoptosis.The morphology of HT-29 changed as nuclear chromosomal condensation and segmentation.Sub-G_1 peak was found with flow cytometry.The expression of caspase-9 and bax gene was enhanced.The expression of bcl-2 gene was weak.Conclusion Apoptosis of HT-29 cell is the key mechanism to ursolic acid's killing action in the treatment of cancer.