Expression and value of VEGF and TGF-β_1 protein in experimental rat after focal cerebral ischemia

Objective To investigate the change of vascular endothelial growth factor (VEGF) and transforming growth factor-beta1(TGF-β 1)expression in rat focal cerebral ischemia-reperfusion modals in different ischemic cerebral areas and at different time,in order to study their relationship and value.Methods The middle cerebral artery ischemia-reperfusion models were establishd with the methords of occluding the middle cerebral artery with nylon thread in rat.There were 36 male SD rats which were divided randomly into control groups,sham-operated,12h,24h,3d,7d post-reperfusion groups.VEGF,TGF-β1expression were examined by immunohistochemistry.Results The expression of VEGF occure at 12 hour after reperfusion,increased at 24h until 3d,and then decreased,but also expressed little at 7d.The expression of TGF-β1 was accorded with VEGF.In operation groups the most marked upregulation occurred in vacular endothelial cells,neurons and gliocytes in the infarct and the border zones.The positive expression in the border zones showed a moderately intense than infarct zones.Conclusion The proteins of VEGF and TGF-β1 are related to the pathophysiologic processes after focal ischemic injury and self-repair.