Experimental study on role of Fufangbiejiaruanganfang in serum prevention and controlling of liver fibrosis and its mechanism

Objective To explore the role of Fufangbiejiaruanganfang in serum prevention and controlling of the liver fibrosis and its mechanism.Methods 45 male SD rats were randomly divided into normal group,model group and Fufangbiejiaruanganpian group( FFBJRGP group) by proportion of 1∶1∶1,and normal group were injected olive oil,3 m L /( kg·d),and distilled water of 1 g /( kg·d) were gavaged for 6 weeks; the model group were subcutaneously injected with 40% CCl4 prepared by olive oil 3 m L /( kg·d) to construct the model of liver fibrosis in rats,and distilled water of1 g /( kg·d) were gavaged for 6 weeks; FFBJRGP group were injected with 40% CCl4 prepared by olive oil 3 m L /( kg·d),and Fufangbiejiaruanganpian of 1 g /( kg · d) were gavaged for 6 consecutive weeks. Related indexes,serum COX-2mRNA,COX-2 protein,α-SMA,TXB2,6-K-PGF1αand TXB2/6-K-PGF1αlevels were compared among the three groups. Results Total protein,AST and ALT levels in model group and FFBJRGP group were significantly higher than those in normal group( P 0. 05),albumin levels were significantly lower than that in normal group( P 0. 05); total protein,AST and ALT levels in FFBJRGP group were significantly lower than those in model group( P 0. 05),albumin level was significantly higher than that in model group( P 0. 05). COX-2 mRNA,COX-2 protein,α-SMA scores in model group and FFBJRGP group were significantly higher than those in normal group( P 0. 05),the above scores in FFBJRGP group were significantly lower than those in model group( P 0. 05). The levels of TXB2 and TXB2/6-K-PGF1αin normal group and FFBJRGP group were significantly lower than those in model group( P 0. 05),there were no statistically significant differences of 6-K-PGF1αlevels in three groups. Conclusion Fufangbiejiaruanganfang could effectively improve hepatic function in rats with hepatic fibrosis,reduce the level of COX-2 in rat liver fibrosis may be one of mechanisms on prevention and treatment of liver fibrosis.