Protective effects of Astragaloside IV on hydrogen peroxide-induced injury of cultured neonatal rat myocardial cells

Objective To investigate the protective effects of Astragaloside Ⅳ(ASⅣ)on cardiomyocytes against hydrogen peroxide(H2O2)-induced injury.Methods Cultured neonatal rat cardiomyocytes were divided into control group;0.1%DMSO solven group;H2O2 group,in which cells were treated with H2O2( 0.2 mmol·L-1) for 24 h;ASⅣ+H2O2 groups,in which cells were pretreated with ASⅣ(with final concentration of 3,10,30 mg·L-1) for 30 min before H2O2 treatment;Astragaloside injection group(150 g·L-1).Morphological changes of myocardial cells damaged for 24 h by 0.2 mmol·L-1 H2O2 under the inverted microscope were observed.The cardiomyocyte viability was analysed by MTT assay;lactate dehydrogenase(LDH) activity,superoxide dismutase(SOD) activity,malonaldehyde(MDA) content and nitric oxide(NO) content were detected in culture media.Results Most of cells in H2O2 group were crenulated,the nucleus were dim and the pseudopodia were disappeared.The degrees of lesion in ASⅣ(3,10,30 mg·L-1) groups were lessened obviously,the nucleus were bright and the the pseudopodia were thinned slightly.Cardiomyocyte viabilities in ASⅣ(3,10,30 mg·L-1) groups were higher than that in H2O2 group(P0.05 or P0.01).LDH activity and MDA content in ASⅣ(10,30 mg·L-1)groups were lower than those in H2O2 group(P0.05 or P0.01),and SOD activity and NO content in ASⅣ(10,30 mg·L-1)groups were higher than those in H2O2 group(P0.05 or P0.01).Conclusion ASⅣ can protect cardiomyocytes from H2O2 injury by improving cell antioxidant ability and increasing NO content.