Effect of Ozone Oxidative Preconditioning on Expression of Toll-like Receptor 4 in Renal Ischemia Reperfusion Injury in Rats

Objective To observe effects of ozone oxidative preconditioning(OzoneOP) on expression of toll-like receptor 4(TLR4) in rats with renal ischemia-reperfusion injury(I/R).Methods Forty-eight rats were randomly divided into sham operated group,sham operated+OzoneOP group,I/R group and I/R+OzoneOP group(n=12 in each group).Rectums of rats with OzoneOP were insufflated Oxygen+Ozone mixed gas 5~5.5 ml(Ozone concentration 50 mg/L,1 mg/kg,1 time a day) 15 days before operation,then the rats' unilateral renal I/R animal models were set up according to requirements of each group.The blood urea nitrogen(BUN),creatinine(Cr),expressions of TLR4 mRNA and TLR4 protein were detected 24 hours later.Results BUN,Cr,contents of TLR4 mRNA and TLR4 protein in I/R group and I/R+OzoneOP group were significantly higher than those of sham operated group and sham operated+OzoneOP group(P0.05),while the above 4 indexes in I/R group were significantly higher than those of I/R+OzoneOP group(P0.05);but the differences between sham operated group and sham operated+OzoneOP group were not statistically significant(P0.05).Conclusion OzoneOP can alleviate renal I/R injury in rats by way of the inhibition of TLR4 expression.