Influence of sodium selenite on expression of p38 mitogen-activated protein kinase and peroxisome proliferator-activated receptor γ in rat mesangial cells line HBZY-1

Objective:To observe the influences of sodium selenite on expression of p38 mitogen-activated protein kinase(p38MAPK) and peroxisome proliferator-activatee receptor γ(PPARγ) in rat mesangial cells line HBZY-1,so as to study the role of p38MAPK and PPARγ in diabetic nephropathy and the mechanism by which sodium selenite prevents diabetic nephropathy.Methods: Rat mesangial cell line HBZY-1 was incubated with high glucose,high insulin,H_(2)O_(2) and advanced glycosylation end products(AGEs) separately before and after HBZY-1 cells were pre-treated with SB203580(p38MAPK special inhibitor)or sodium selenite.Cells receiving no stimulation were taken as control.The expression of p38MAPK protein and PPARγ mRNA was detected respectively by immunohistochemistry assay and RT-PCR in all groups and the results were compared.Results: High glucose,high insulin,H_(2)O_(2) and AGEs all activated p38MAPK,increased phospho-p38MAPK expression and decreased the expression of PPARγ mRNA in rat mesangial cells line HBZY-1.The expressions of phospho-p38MAPK protein was markedly inhibited by sodium selenite,while the expression of PPARγ mRNA was significantly increased by SB203580 or sodium selenite in rat mesangial cells lines HBZY-1(P0.01).Conclusion: p38MAPK may antagonize the expression of PPARγ in rat mesangial cells lines HBZY-1.Sodium selenite,with a similar effect to the agonist of PPARγ,can obviously increase the expression of PPARγ.