Research on distribution,serogroups and virulence genes of Vibrio parahaemolyticus in different samples

Objective To understand the distribution of Vibrio parahaemolyticus from diarrhea patients and aquatic products in Jianggan district,and explore the similarities and differences of the serogroups and virulence genes of these Vibrio parahaemolyticus from different sources. Methods The Vibrio parahaemolyticus was isolated and identified according to GB / T4789. 7-2008,and the real-time PCR was used to detect the virulence gene tdh and trh from these strains. Results The detection rates of Vibrio parahaemolyticus in sporadic diarrhea patients,food poisoning patients and aquatic products were 16. 36%( 45 /275),85. 71%( 18 /21) and 39. 69%( 102 /257). Clinical strains( including sporadic and outbreaks patients) were concentrated on serum group O3,O1 and O4,and the strains from aquatic products were concentrated on serum group O1,O10,O2,O5 and O11. The detection rates of tdh and trh from clinical strains were 96. 88%( 62 /64),1. 56%( 1 /64),while the tdh and trh were not detected in strains from aquatic product. Conclusion Vibrio parahaemolyticus is the main pathogen of sporadic and outbreak diarrhea patients in Jianggan district. Retail aquatic products were contaminated heavily by Vibrio parahaemolyticus. The serogroups and virulence genes of strains had large difference between clinical patients and aquatic products.