Effect of siRNA Inhibited COX-2 Gene on Biological Characteristics of MG-63 Cells

Objective Through establishing the small interfering RNA(siRNA)to decrease the expression of MG-63 osteosarcoma cell's cyclooxygenase-2(COX-2)gene,and further research the influence and molecular mechanism of the power for proliferation,invasion and migration for MG-63 osteosarcoma cell.Methods Designed the targeted interference COX-2 gene's siRNA,transfected MG-63 osteosarcoma cell by the lipidosome,so as to suppress the expression of COX-2 gene in MG-63 osteosarcoma cell,then we used the MTT colorimetric method,and Transwell small room experiment to research the influence to the power of proliferation,invasion and migration for MG-63 osteosarcoma cell.Using RFQ-PCR and Western blot to detect the expression of the MG-63 osteosarcoma cell invasive related factor matrix metal enzyme(MMP-9)expression and the expression of vascular endothelial growth factor(VEGF)from the gene level and the protein level.Results After transfecting the MG-63 osteosarcoma cells,comparing the experiment group with the negative control group and the blank control group,through the RFQ-PCR and Western blot detecting COX-2 gene's expression about 90%(P 0.05),MTT detecting the MG-63 osteosarcoma cells'multiplication capacity which is suppressed obviously(P 0.05),using Transwell experiment to detect MG-63 osteosarcoma cells'invasion,migration ability decrease significantly(P 0.05),through the RFQ-PCR,Western blot detecting the invasive related factor MMP-9 and vascular endothelial growth factor VEGF's mRNA and protein expression reduced(P 0.05).The blank control group and the negative control group had no significant change,there was no statistically significant difference(P 0.05).Conclusion COX-2 siRNA can significant suppress the COX-2 gene's expression in people's MG-63 osteosarcoma cell,and then further induce MG-63 osteosarcoma cell's power of proliferation,invasion and migration.