Effects of Doxycycline Hydrochloride and Lipiodol on the Apoptosis and Proliferation of VX2 Hepatic Tumors and Liver Function of Rabbits

OBJECTIVE:To investigate the effect of doxycycline hydrochloride(DOXY) and lipiodol on the apoptosis and proliferation of VX2 hepatic tumor and liver function of rabbits.METHODS:VX2 implanted hepatic tumor model was established and 2 weeks later they were randomly divided into group A(normal saline),group B(lipiodol 0.3 mL·kg-1),group C(adriamycin 2 mg·kg-1+lipiodol 0.3 mL·kg-1) and group D(DOXY 3 mg·kg-1+lipiodol 0.3 mL·kg-1) via catheter inserted into the arteria cruralis and hepatic artery of rabbit.The levels of AST and ALT in serum were respectively tested at 1 day before treatment,and 4,7 days after treatment.The apoptosis index(AI) of tumor cells and the expressions of Caspase-3 were measured,and proliferation index(PI) of proliferating cells was also detected.RESULTS:Compared with group A,the contents of AST and ALT increased significantly in group B,C and D 4 days after treatment(P0.05);7 days after treatment,the contents of AST and ALT tended to decrease,but the contents of AST were still higher in 3 groups(P0.05),and the content of ALT higher in group C(P0.05);AI and Caspase-3 expression of tumors in 3 groups increased significantly(P0.05),and PI of tumor decreased significantly(P0.05).Compared with group B,AST and ALT contents increased significantly in group C 4 and 7 days after treatment(P0.05);AI and Caspase-3 expression of group D increased significantly(P0.05),while PI decreased significantly(P0.05).CONCLUSION:DOXY and lipiodol have nearly no significant damage on liver function,and can promote the apoptosis of tumor cells and reduce proliferation of it.DOXY promote the apoptosis of tumor cells which may be associated with the up-regulation of Caspase-3 expression.