2004•Journal of Shantou University Medical CollegeRequires access

Preparation of Influenza A Virus Antiserum from Chickens with Specific Pathogen Free

Kangsheng Li, Hku Joint

Abstract

Objective:To make antiserum against influenza A virus for influenza virus subtype identification.Metho-ds:Healthy, adult chickens with specific pathogen free(SPF)were immunized with two different H11-subtype influenza A virus strains and stimulated to produce antiserum. Hemagglutinin inhibition test was performed to detect titer of antise- rum. Results:Antibody against H11-subtype influenza A virus could be detected in all immunized SPF chickens. However, there was significant difference of antibody titer between two groups.Conclusion:The method adopted currently is convenient and specific. The antibody produced by this method can be used for influenza A virus subtype identification.

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Key concepts: Virology, Antiserum, Hemagglutinin (influenza), Virus, Titer, Pathogen, Specific-pathogen-free, Biology