Construction and expression of eukaryotic fluorescent expressing vector of VEGF-C gene in CHO cell

Objective:To construct eukaryotic expressing vector of VEGF-C gene with EGFP gene and test its expression in CHO cells.Methods:cDNA of EGFP in plasmid of pEGFP-1 was inserted into eukaryotic expressing vector pcDNA3.1/VEGF-C by DNA recombinant technology and further transfected into CHO cell with lipofectin.Expression of EGFP in transfectants was examined by fluorescence microscope.The expression of VEGF-C gene was examined by RT-PCR.Results:Agarose gel electrophoresis proved the insert fragment was EGFP gene.It was proved further by DNA sequence.After being selected by G418,one clone was obtained.mRNA of exogenous VEGF-C gene could be detected by RT-PCR.Conclusion:The eukaryotic expressing vector of VEGF-C with EGFP gene was constructed successfully and could be expressed in CHO cell.