Optimization of aqueous enzymatic extraction of peony seed oil by response surface methodology

Peony seed oil was extracted by aqueous enzymatic method. On the basis of single factor experiment,the aqueous enzymatic extraction process of peony seed oil was optimized by the Box-Behnken design of response surface methodology,and the fatty acid composition of peony seed oil was analyzed by GC-MS. The results showed that the optimal extraction conditions were obtained as follows: ratio of solid to liquid 1∶ 5. 4,enzymatic hydrolysis temperature 52 ℃,enzymatic hydrolysis pH 10. 3,dosage of enzyme550 U /g. Under these conditions,the yield of peony seed oil reached 23. 25%. Linolenic acid,linoleic acid,palmitic acid and stearic acid were the main four kinds of fatty acids in peony seed oil,and their contents were 58. 16%,24. 05%,12. 33% and 3. 56%,respectively.