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2006•Yati yasui yazhoubingxue zazhiRequires access

Icariin enhances the proliferation and expression of osteoprotegerin of human periodontal ligament cells in vitro

Qintao Wang

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Abstract

AIM:To investigate the effects of icariin on the proliferation and expression of osteoprotegerin(OPG) mRNA of the human periodontal ligament cells(HPDLC).METHODS:HPDLC were cultured in vitro and stimulated with icariin winh different concentrations(0.001～10 μg/mL) for 0,24,48,72 and 96 hours.The ability of proliferation of HPDLC was detected by MTT method and RT-PCR was used to examine the expression of OPG mRNA in HPDLC.RESULTS:The proliferation and expression of OPG mRNA of HPDLC were stimulated by icariin((P0.05)),and the most efficient concentration was 0.1 μg/mL(P0.01).CONCLUSION:Icariin may upregulate the expression of OPG mRNA in HPDLC by stimulating its proliferation.

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AIM:To investigate the effects of icariin on the proliferation and expression of osteoprotegerin(OPG) mRNA of the human periodontal ligament cells(HPDLC).METHODS:HPDLC were cultured in vitro and stimulated with icariin winh different concentrations(0.001～10 μg/mL) for 0,24,48,72 and 96 hours.The ability of proliferation of HPDLC was detected by MTT method and RT-PCR was used to examine the expression of OPG mRNA in HPDLC.RESULTS:The proliferation and expression of OPG mRNA of HPDLC were stimulated by icariin((P0.05)),and the most efficient concentration was 0.1 μg/mL(P0.01).CONCLUSION:Icariin may upregulate the expression of OPG mRNA in HPDLC by stimulating its proliferation.

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Available abstract

AIM:To investigate the effects of icariin on the proliferation and expression of osteoprotegerin(OPG) mRNA of the human periodontal ligament cells(HPDLC).METHODS:HPDLC were cultured in vitro and stimulated with icariin winh different concentrations(0.001～10 μg/mL) for 0,24,48,72 and 96 hours.The ability of proliferation of HPDLC was detected by MTT method and RT-PCR was used to examine the expression of OPG mRNA in HPDLC.RESULTS:The proliferation and expression of OPG mRNA of HPDLC were stimulated by icariin((P0.05)),and the most efficient concentration was 0.1 μg/mL(P0.01).CONCLUSION:Icariin may upregulate the expression of OPG mRNA in HPDLC by stimulating its proliferation.

Key concepts: Icariin, Osteoprotegerin, Periodontal fiber, Chemistry, In vitro, Downregulation and upregulation, Messenger RNA, Cell biology

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Icariin enhances the proliferation and expression of osteoprotegerin of human periodontal ligament cells in vitro — Research Paper | ScholarLens