Determination of Soybean Isoflavones Content by High-Performance Liquid Chromatographic Method

The five soybean isoflavones components including Genistein,Genistin,Daidein,Daidzin and Glycitin were determined and separated by the established HPLC method,and isoflavones in different tissues and different grow stages of embryo were measured.The HPLC condition we have established:an phenomenex C18column(150 mm×4.6 mm,5.0 μm)was applied;the mobile phase was MeOH ∶H2O=30 ∶70;the temperature of column was 40℃;the flow rate was 10 mL·min-1;detective wavelength was set at 254 nm.The peak area of five isoflavones had a good linearity with a correlation coefficient of 0.9994-0.9998,and the recoveries of standard spiling of isoflavones were in the range of 99.29%-100.76% with RSD less than 3%.This method was suitable for determination of isoflavones in soybean and other cereal products.The isoflavone content among soybean varieties was Jilin 32 GR8836Jidadou 2 Pingan 8 XiaoheidouJilin 47Jilin 35Jidadou 2,and soybean isoflavone content of Jilin 32 was that in seeds60 d embryos50 d embryos40 d embryosflowerspods20 d embryos30 d embryosleavesrootsstems.