2012•Unpublished venueRequires access

A novel Bacillus thuringiensis strain with broad spectrum and high toxicity against lepidoptera vegetable pests.

Ge DongHua, Junping Su, Ping Song, Ziyan Nangong, Qinying Wang

Abstract

Bacillus thuringiensis(Bt) strain MB-15,isolated from the soil in Hebei Province,was high toxic against Lepidoptera vegetable pests.Compared with Bt HD-1,the toxicities of Bt MB-15 against Helicoverpa armigera,Spodoptera exigua,Plutella xylostella,Spodoptera litura and Pieris rapae all were higher than that of Bt HD-1 by bioassay in lab.The supernatant of Bt MB-15 fermentation could remarkably enhance the insecticidal activity of Bt MB-15 crystal protein.The bipyramidal crystals were observed by microscopic observation in Bt MB-15 strain.SDS-PAGE analysis revealed that Bt MB-15 produced crystal proteins of 130.0 kDa and 65.0 kDa.Bt MB-15 contained cry1Ac,cry2Aa,cry1I and vip3Aa gene by PCR-RFLP analysis.The results showed that Bt MB-15 could have the potential and commercial development value against Lepidoptera vegetable pests.

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Key concepts: Bacillus thuringiensis, Cry1Ac, Plutella, Lepidoptera genitalia, Plutellidae, Pieris rapae, Helicoverpa armigera, Biology