Isolation,Identification and Cultural Character of a Strain of Purple Nonsulfur Photosynthetic Bacteria

[Objective]The research aimed to obtain a new bacteria source of functional photosynthetic bacteria. [Method]A strain of purple nonsulfur photosynthetic bacteria GB-1 was isolated and purified from the sediment of fishpond in Maoming suburbs. The classification and identification of purple nonsulfur photosynthetic bacteria GB-1 was carried out by the observation of cell morphology,the cell coloring matter absorption spectrum determination and the study of physiological and biochemical characteristics. Meanwhile,the cultural character of the strain GB-1 was subsequently investigated. [Result]The strain GB-1 was identified as Rhodopseudomonas palustris. The optimum conditions for its growth were studied as follows: the best carbon source was a small molecule organic acid,growth temperature was 32- 35 ℃,p H was 8-9,Na Cl concentration was less than 1%,and the condition of illumination was incandescent light bulb and micro-aerobic. [Conclusion]Halotolerated ability of GB-1 were 5%. After the cultivation for 7 d,the removal rates for ammonia nitrogen and nitrite by the strain GB-1 was up to 57. 71% and 47. 45%,respectively. It appeared broad application prospects in the treatment of eutrophic water.