Effect of butylphthalide on memory function and hippocampal neuronal metabolites in vascular dementia rats

Objective To explore the hippocampal metabolic changes of butylphthalide intervened vasculardementia( VD) rats and the functions of butylphthalide on learning and memory improvement. Methods 10 rats were randomly selected from 90 healthy male SD rats to be sham operation group,and the remaining 80 rats were given 2VO surgery to make VD models. 48 rats survived after 8 weeks,and then randomly divided into 4 groups:VD model group,NBP low,medium,high dose group,each had 12 rats. Rats of NBP low,medium,high dose group were injected NBP 12. 5,25,50 mg /( kg·d),respectively. VD model group and sham operation group were injected with the same volume of 0. 5% Tween-80 solution,all rats were continuous injection of 4 weeks. Behavior test was investigated by place navigation test and spatial probe test. Changes of neuronal metabolites were detected by1H-MRS. Results Behavior test results indicated that rats' learning and memory ability were proportional to the DBP dose: 1 ~ 5 days,the average escape latency in NBP medium,high dose group were significantly lower than model group( P 0. 05),and spatial probe test results indicated that DBP high dose group could significantly improve the learning ability of rats;1H-MRS detection results showed that compared with after administration of VD with those in the model group,NAA / Cr,Cho / Cr and m I / Cr in medium dose group and high dose group,the differences were statistical significance( P 0. 05),but no statistical significance in NBP low dose group. The results indicated that the middle dose group and high dose group of glial cells in hippocampus hyperplasia had obvious effect,and low dose NBP group had no obvious effect. Conclusion Butylphthalide can improve the ability of learning and memory in rats with vascular dementia,and regulate hippocampal neuronal metabolites to improve function of VD.