Effects of high salt diet on oxidative stress and Na~+-K~+-ATPase and Ca~(2+)-ATPase activities in cerebral cortex of Wistar rats

Objective To study the effects of high salt diet on oxidative stress and Na+-K+-ATPase and Ca2 +-ATPase activities and the mRNA expression in cerebral cortex of Wistar rats. Methods Wistar rats were divided into normal salt control( NS)( n = 13),high salt( 8%) model( HS,n = 24) and high salt + telmisartan( HS + Tel) groups( n = 12). Tail-cuff artery pressure was determined every 2 weeks.Values of SOD activity and MDA content were determined by colorimetry. The activities and mRNA levels of Na+-K+-ATPase and Ca2 +-ATPase in cerebral cortex were determined by enzyme colorimetry and real-time polymerase chain reaction( PCR),respectively. Results Compared with those of NS group,activity of SOD was decreased and MDA content was increased. The activities of Na+-K+-ATPase and Ca2 +-ATPase were significantly reduced,whereas the mRNA expressions of NAPDH,Na+-K+-ATPase α1 subunit and PMCA1 were increased.After treated with telmisartan,the activities of Na+-K+-ATPase and Ca2 +-ATPase became much higher compared with those of HS group.Conclusions Long-term high-salt diet could lead to oxidative stress and down-regulat activities of Na+-K+-ATPase and Ca2 +-ATPase in cerebral cortex.