Ground Research of Space Microcarrier Cell Culture Techniques

Two cell lines L929 and CHO were cultured in cell bioreactor with microcarrier suspension culture system. The optimum conditions for cell proliferation were studied.The result showed that microcarrier density 2 mg/ml,an even higher cell moculation density,sernm density abeut 10% were suitable for cells to grow. The cell production under such conditions with microcarrier suspension techniques were 26 times more than that with mono-layer culture. Scale-up of culture volume and beads to beads transfer techniques were devel-oped and new bioreactor were explored to be used in space.