Analgesic effect of Qufeng Xitong Wan on experimental pain in mice

Objective To investigate the analgesic effect of Qufeng Xitong Wan(QFXTW)and to provide an experimental basis for its clinical use.Methods Experimental pain models of mice were established to investigate the analgesic effect of QFXTW.①Writhing test:One hundred Kunming mice were divided into 5 groups(n=20 in each group,10 male and 10 female).The mice was gavaged with physiological saline 10 ml/kg in control group,with QFXTW 0.36,1.08,3.24 g/kg in QFXTW groups,and with aspirin 0.4 g/kg in aspirin group(positive control group).Drugs were given once a day for 10 d.After the last administration,the writhing response times of mice were recorded during 5-20 min after intraperitoneal injection of acetic acid.②Hot plate test:A hot plate with a temperature of(55±1) ℃ was used to test the pain threshold of mice.The time from putting on the hot plate to licking postpedes was defined as pain threshold time of mice.Fifty female mice with pain threshold times from 5 s to 30 s were divided into 5 groups as described above.The pain threshold time was recorded after drug administration for 10 d.Results ①Compared with control group,the writhing response times of mice significantly decreased with inhibition rates of 25.4%,30.0% and 43.4% after gavaged with QFXTW 0.36,1.08 and 3.24 g/kg for 10 d(P0.05),respectively.②Pain threshold was not significantly different among the groups before drug administration(P0.05).After gavaged with QFXTW 0.36,1.08 and 3.24 g/kg for 10 d,the pain threshold significantly prolonged compared with that of coutrol group(P0.05).Conclusion QFXTW has an analgesic effect on experimental pain of mice.