Conditions of high efficient electroporation transformation of Pichia pastoris

Objective A high efficient method of electroporation transformation was established to provide the basis for expressing the functional genes of marine drugs in Pichia pastoris.Methods The method used to Extraneous DNA was transformed into Pichia pastoris genome by electroporation transformation.The main factors that affect the transformation efficiency were explored and optimized.Results The optimal conditions for electroporation transformation of Pichia pastoris were obtained with the maximum transformation efficiency of 36 transformants/μg plasmid DNA when the intermediate logarithmic phase yeast cells were used for competent cells,and the electroporations were under the conditions of voltage of 1.5 kV,0.15 g·L-1 plasmid DNA and 0.2 cm cuvettes.All the transformants were confirmed to be positive clones.Conclusion The highly effective transformation technique was obtained through optimizing the electroporation transformation conditions.