Detection of intracellular calcium concentration on pulmonary artery smooth muscle cells on rats

OBJECTIVE To detect intracellular calcium concentration((Ca2+)i) in fresh culturing pulmonary artery smooth muscle cells(PASMCs) on rats.METHDOS The pulmonary arteries of rats were isolated on microscope and were digested with low-Ca2+ HBSS's solution(37℃),containing collagenaseⅠ(1750U·mL-1) and papain(9.5U·mL-1).(Ca2+)i of PASMCs were measured using real-time cellular fluorescence imaging technique.RESULTS Enough PASMCs for experiments can been obtained within 18～24h,and the enhancement(Ca2+)i of PASMCs can been induced by both 10μmol·L-1 cyclopiazonic acid and 10μmol·L-1 5-HT.CONCLUSION This isolated and cultured method is convenient and useful.The PASMCs that the structure and function was normal could be applied to real-time cellular flourescence imaging technique experiment and the research of PASMCs signal transduction function.