Study on Biflavonoids from Selaginella moellendorfii Hieron.

OBJECTIVE To study biflavonoids in Selaginella moellendorfii Hieron. METHODS The biflavonoids were isolated and purified through column chromatography on silica gel and Sephadex LH-20 from the ethyl acetate extract of Selaginella moellendorfii Hieron.,and their structures were elucidated with physicochemical methods and spectroscopic methods including 1H-NMR,13C-NMR. RESULTS Six biflavone compounds were isolated from Selaginella moellendorfii Hieron,their structures are identified as 7,4',7''-tri-O-methylrobustaflavone(Ⅰ),7,7''-di-O-methylrobustaflavone(Ⅱ),4'-O-methylrobustaflavone(Ⅲ), robustaflavone(Ⅳ),hinokiflavone(Ⅴ), amentoflavone(Ⅵ). CONCLUSION CompoundⅠ～Ⅴ were isolated from Selaginella moellendorfii Hieron. for the first time,among them compound Ⅰ and Ⅱ were obtained from the Selaginella genus for the first time.