Effect of TRAIL on sensitivity of human glioma cell line to chemotherapy

OBJECTIVE:To investigate the effect of TRAIL on the sensitivity of human glioma cell line to chemotherapy and study the principle of induction of apoptosis by TRAIL.METHODS:After the culture of human U251 glioma cell line, different doses of TRAIL and VM26 were acted on the glioma cell. Viabilities of groups of TRAIL and VM26 were examined by MTT assay, components of DNA were examined by analytical method of DNA fragmentation, then sub-poison doses of TRAIL and VM26 were acted on the same well of glioma cell. The apoptosis rates of three experimental groups were examined by FCM. RESULTS:The apoptosis rates of group of 100 ng/mL TRAIL, 2 μg/mL VM26, and the TRAIL combined with VM26 were 19%, 15% and 60% respectively. The apoptosis rate of glioma cell induced by VM26 was significantly lower than that of TRAIL combined with VM26,P=0.004. Typical DNA fragmentation appeared in the group of TRAIL and combination after DNA ladder.CONCLUSION:TRAIL could increase the sensitivity of human glioma cell line to chemotherapy.