Expression and Purification of the Recombinant Protective Antigen of Bacillus anthracis in Escherichia coli

The primers specific for the protective antigen(PA) of Bacillus anthracis coding sequence was designed and synthesized. The PA gene was cloned from pOX1 plasmid by using PCR and inserted into vector pET-28a to obtain the recombinant expressing plasmind pET-28a-PA. The restriction enzyme analysis and DNA sequence detection confirmed that the inserted fragment of clone pET-28a-PA is the mature PA coding sequence. The recombinant DNA was transformed into the host cells E.coli BL21(DE3). The BL21(DE3) with pET-PA was induced with IPTG. The fusion protein effective expressed. The result of Western-blot showed that this fusion protein reacted specifically to the monoclonal antibodies to PA.