Multiresidue determination of organochlorine pesticides in honey by gas chromatography-mass spectrometry

An analytical multiresidue method for the simultaneous determination of 12 organochlorines pesticides in honey was developed.Pesticides were extracted from honey with hexane/ethyl acetate(5/1,V/V),in an ultrasonic bath and cleaned up on a Florisil column,then were determined by using a gas chromatography-mass spectrometry operated in electron ionization mode and quantified in selective ion monitoring mode,and with PCB103 as internal standard.Meanwhile,fracture mechanism of some organochlorines electronic ionization mass spectrometry were evaluated.Recovery studies were performed at 10,50 and 200 μg/kg fortification levels for each pesticide,and the recoveries ranged from 80% to 112% with a relative standard deviation between 0.4% and 9.8% for the different pesticides.The detection limit of the method was less than 1.0 μg/kg for the most of pesticides.The developed method was linear over the range assayed,10～500 μg/L,with correlation coefficients 0.996.Finally,the developed analytical method has been successfully applied to the determination of pesticides in several honey samples.