Studies on stable transfection of TGFβ1 sense and anti-sense gene in human embryolung fibroblasts irradiated by ~(60)Co-rays

Human embryo lung fibro blasts(HELFs) irradiated with 5 Gy 60Co γ-rays were transfected with the expression vector pMAMneo-TGFβ1 or pMAMneo-anti-TGFβ1 that encode the human TGFβ1 sense or antisense gene.The transfected cells were elected by G418 resistance. The chromosome DNA were extracted. Production of 276bp was amplified from chromosome DNA by a PCR method of neo-specific primer. DNA dot blot analysis showed that cells transfected pMAMneo-TGFβ1 or pMAMneo-antiTGFβ1 hybridized with Dig-labeling neo-specfic probe. Results indicated HELFs after irradiation of 60 Co γ-rays could be transfected stablly using a polycation lipid-mediated method.