Analysis on eggshell protein changes after silkworm eggs soaked by HCl

Diapause and non-diapause eggs were laid by the temperature and humidity regulation in the egg and larvae feeding stage of 932 variety. Diapause eggs by HCl treatment were observed more visible eggshell cracks than the control by scanning electron microscope. The total proteins of diapause eggs,diapause eggs by HCl treatment and nondiapause eggs were compared using SDS-PAGE electrophoresis. Small molecular proteins below 20 ku decreased in the proteins of diapause eggs by HCl treatment. The small molecular proteins were identified by shotgun analysis. The Er B.1,Er B.2,Er B.3,Hc-A and Hc-B eggshell proteins were detected in the diapause and non-diapause eggs,but were not detected in the diapause eggs by HCl treatment. It is speculated that the diapause eggs by HCl treatment may cause the degradation of the five eggshell proteins.