Effects of exercise on IL-2,IL-2R expression and CD4,CD8 activity in blood of RBP4-induced insulin resistance rats

Objective: To study the effects of exercise on IL-2,IL-2R expression and CD4,CD8activity in blood of RBP4-induced insulin resistance rats. Methods: 8-week-old SD male rats were established by intraperitoneal injection with recombination RBP4 3μg / g,2 times per day for 4weeks. Model rats were divided into RBP4 + exercise group( RE),RBP4 + rest group( RR),and normal control group( C). RE group rata swam without load,60 min per day for four weeks. IHC and western blotting were used to detect serum RBP4 and HOMA-IR. ELISA was used to detect serum IL-2、IL-2R expression. S-P method was used to detect activity of CD4 and CD8 in the blood. Results: Serum RBP4 and HOMA-IR in RE group were significantly lower than RR group.Comparing to RR group,CD4 increased( P 0. 05) while CD8 decreased( P 0. 05) in RE group,and CD4 / CD8 ratio increased obviously( P 0. 01) in RR group. IL-2 expression grew obviously( P 0. 05) while IL-2R reduced( P 0. 05),so the IL-2 / IL-2R ratio increased obviously( P 0. 01).Thymus index( P 0. 05) and spleen index( P 0. 01) in RE group rise distinctly when compare with RR group. Conclusion: Immune function of RBP4-induced insulin resistance rats had been weakend. But exercise can strengthen the immune function of them.