Effect of Nerve Regeneration Factor on GAP-43 and NF-L Gene Expression in Dorsal Roots Ganglia Cell

AIM To Detect the varying of gene expression in cultured dorsal root ganglion cells treated by Nerve Regeneration Factor and explore its molecular mechanism promoting to nerve growth. METHODS By RT PCR, the change of gene expression in GAP 43 and NF L on the cultured DRG cells with Nerve Regeneration Factor were studied during 4 h, 12 h, 24 h. RESULTS The expression of GAP 43 and NF L were increased by Nerve Regeneration Factor on DRG′s culture. CONCLUSION This study indicated Nerve Regeneration Factor may up regulate the gene expression associated nerve growth on cultured nervous cells.