Effect of SBEF-Ca on Bone Mineral Density and Biochemical Parameters of Bone Metabolism in Rats with Osteoporosis

Objective The purpose of this study was to investigate the preventive and therapeutic role of sheep bone enzymatic fermentation-calcium chelate(SBEF-Ca) against osteoporosis.Methods Forty 3.5-month-old Sprague-Dawley rats were randomly divided into normal group(sham + water,ig),model control group(ovariectomized + water,ig),and three dosage groups(ovariectomized + SBEF-Ca,ig).From 12 days after ovariectomy,the rats were treated with water or SBEF-Ca intragastically,respectively,for 11 weeks.Then the femoral bone mineral density(BMD) and femoral length,as well as the main parameters of bone metabolism were measured.Results Both the femoral BMD and the length were significantly decreased in the model control group,and all the dosage groups reversed the declining tendency and brought them to the level of normal group.BMD of the high-dosage group was markedly higher than that of model group and the other two dosage groups.There were no statistically significant differences in the femur length among dosage groups.The serum Ca and P levels of all rats had no significant differences.An apparent rise of alkaline phosphatase(ALP) and bone gla protein(BGP) occurred in the model control group,but these two parameters of all dosage groups had no significant differences compared with those of the normal group(P 0.05).ALP level of the high-dose group was significantly lower than that of the low and medium dosage groups.The BGP levels of the medium and highdosage groups were significantly lower than that of the low dose group.Conclusion All the low,medium and high dosages of SBF-Ca show preventive effect on the occurrence of postmenopausal osteoporosis in rats.