Impact of Low Temperature Acclimation on Soluble Proteins and Soluble Sugars in Scylla serrata

Mud crab,Scylla serrata,is an important commercial crustacea distributing along the seacoast in the southeast of China.As water temperature fluctuates,physiological reactions correspond to appear.Soluble proteins and soluble sugars play an important role in adaptation of physiological metabolism for low temperature.In this study,biochemical methods were used to measure soluble proteins and soluble sugars in gill,hepatopancreas and muscle of S.serrata acclimatized at low temperature.The results showed that soluble proteins content in gill and muscle gradually decreased with decrease of acclimation temperature.Soluble proteins content at 15℃ was higher than that at 27℃,but soluble proteins content at 10℃ and 5℃ was lower than that at 27℃.For hepatopancreas,soluble proteins content gradually increased with decrease of acclimation temperature,and soluble proteins content in each group of three acclimation group was higher than that at 27 ℃.For three acclimation temperature,soluble sugars content in gill,hepatopancreas and muscle decreased gradually with decrease of acclimation temperature,and soluble sugars content at 15℃ was higher than that at 27℃.The above results indicatel that increase of soluble proteins and soluble sugars under 15℃ acclimation is a positive physiological reaction;decrease under 5℃ acclimation(excluding soluble proteins in hepatopancreas) is caused by decrement of soluble proteins and soluble sugars in maintaining physiological homostasis at extra low temperature.In various tissues,difference of soluble proteins and soluble sugars changes cansed by the difference of physiological function and metabolism regulation.