The experimental study on the improved method of Schwann cells' passage culture

Objective: To obtain highly purified and large amount of Scs by improved passage culture for nerve tissue engineering. Methods: Four to five-day-old SD rat's sciatic nerve and brachial plexus were removed, double enzyme digest and single enzyme digest were adopted to primary culture of Scs respectively . Five-seven days later, Scs were purified by single enzyme digest and centrifuge quickly. Results: It was manifested that purification of Scs cultured by improved passage cultural method reached 98%(P0.001). The period of SCs' growth and proliferation checked by MTT extended 1-2 weeks compared with traditional method. Conclusions: Contrasting with the traditional method , this method saves times and avoid effecting on Scs from other substances (Ara-c,etc.), meanwhile activity and purification of Scs will be improved obviously.