Effects of dexmedetomidine on spinal cord ischemia-reperfusion injury in rabbits proinflammatory cytokine expression

Objective To evaluate of dexmedetomidine in rabbit spinal cord ischemia reperfusion injury proinflammatory cytokine tumor necrosis factor-α( TNF-α),interleukin-1β( IL-1β) effect. Methods Twenty-four New Zealand white rabbits were randomly divided into three groups: sham operated group( n = 8),model group( n = 8) and experimental group( n = 8). The rabbit spinal cord ischemia again perfusion model was established,before ischemia 30 min vein infusion of dexmedetomidine 2. 5 μg·kg~(-1)until reperfusion before in experimental group.The model group and sham operation group,and the same time was pumped into the same amount of normal saline. After reperfusion,6,12,24 and 48 h were used to evaluate the motor function of the hind limbs of the rabbits by Tarlov method. The expression levels of TNF-α,IL-1βat the time of 6,12,24,48 h were detected after surgery. The TNF-α,IL-1β,superoxide dismutase( SOD) and malondialdehyde( MDA)were detected and the pathological changes were observed by HE staining and 48 h staining. Results Compared with the sham operation group,the Tarlov score of the model group and the experimental group wasdecreased,serum and spinal cord TNF-α,IL-1β increased,MDA,SOD decreased( all P 0. 05),HE staining pathological damage aggravated. Compared with the model group,the Tarlov score of the experimental group was higher,serum and spinal cord TNF--α,IL-1β decreased,spinal cord MDA and SOD decreased( all P 0. 05). The pathological damage of HE staining was decreased. Conclusion Dexmedetomidine can alleviate the rabbit spinal cord ischemia reperfusion injury,its mechanism may be associated with proinflammatory factor.