2005•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access
Rapid confirmation of the transgenic wheat lines by polymerase chain reaction(PCR)
Meng Chaomin, Xiaodong Zhang, Tianyou Chen
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Abstract
A new rapid DNA extraction method was developed for transgenic wheats identified by PCR.The result showed that this method was convenient,quick and effective.The quality of DNA extracted using this method was suitable for PCR analysis.An optimal reaction sytstem suitable for alien lysine-rich gene PCR was also established in this research:10×Reaction Buffer 2.5 μL,MgCl_2 25 mmol/L,dNTPs(2.5) mmol/L,Genmonic DNA 30-60 ng,primers 1.2 μmol/L,Taq enzyme 1 U,ddH_2O to 25 μL.