Development of method for Enterobacter sakazakii based by PCR in powdered milk.

[Objective] To develop a PCR assay for detection of Enterobacter sakazakii in powdered milk.[Methods] Based on the zpx gene sequence of Enterobacter sakazakii to establish PCR assay,and to verify the specificity and sensitivity.Simulated samples and actual milk samples were tested.[Results] The sensitivity of PCR assay was 102cfu / ml in pure culture.Expected fragment(180bp) were produced from 6 strains of Enterobacter sakazakii after PCR amplification,but not from 31 strains of other bacteria.Enterobacter sakazakii were detected in simulated samples after pre-enrichment.The detection result of actual milk samples showed that there was a good consistency between the PCR assay and conventional method.[Conclusion] This PCR assay is accurate,sensitive,reliable,fast and easy.It should be used widely to detect Enterobacter sakazakii in powdered milk.