Effects of Silencing Migration Inhibitory Factor Gene by RNA Interference on Expression of EMT-associated Protein in Human Cervical Carcinoma SiHa Cell

[Purpose] To investigate the effect of RNA interference(RNAi) targeting macrophage migration inhibitory factor(MIF) on epithelial-mesenchymal transition(EMT)-associated proteins in human cervical carcinoma SiHa cells.[Methods] Recombinant eukaryotic expression plasmid pGenesil-MIF siRNA was constructed and identified,then transfected into human cervical cancer cells SiHa using a liposome approach. Real-time PCR was used to detect the silencing efficacy of MIF mRNA and the expression of E-cadherin、Vimentin mRNA.The protein expression of E-cadherin and Vimentin was detected by immunocytochemistry. [Results] The recombinant plasmid pEGFP-N1-MIF was constructed successfully and transfected into SiHa cells. In experimental group,the mRNA expression of Vimentin decreased,whereas E-cadherin expression increased. Immunocytochemical staining showed that expression of E-cadherin increased,whereas Vimentin expression decreased. [Conclusion] RNA interference of MIF suppresses epithelialmesenchymal transition in SiHa cells by decreasing the expression of E-cadherin and increasing the expression of Vimentin.