A Multiplex PCR for Detection and Identification of Brucella abortus and Brucella suis

A multiplex PCR method was developed for detection and identification of Brucella abortus and Brucella suis.Primers A1/A2 or S1/S2 specific for Brucella abortus or Brucella suis,respectively,were designed in the PCR.A fragment of 424 bp was amplified from Brucella abortus genomic DNA,and a fragment of 535 bp from Brucella suis DNA in the PCR,and two fragments of 424 bp and 535 bp were simultaneously amplified from mixed DNA samples of Brucella abortus and Brucella suis.No amplification was achieved for Corynebacterium,Pheasianus colchicus linnaeus,Mycobacterium avium,or Mycobacterium bovis BCG.It could detect 10/50 L PCR reaction of Brucella abortus or Brucella suis.The PCR can be used to effectively detect and identify Brucella abortus and Brucella suis.