Study on the correlation between integrated state of HPV-16 and the occurrence of cervical cancer

Objective:To explore the role of HPV-16 integration in the occurrence and development of cervical cancer by measuring the prevalence of HPV-16 in patients with cervical cancer and the existential state of HPV in cervical lesions of different grades. Methods:A universal primer of HPV and a specific primer of HPV-16 were used to detect the infection rate of HPV-16 in cervical cancer samples,quantitative PCR was adopted to detect the existential state of HPV in HPV-16 infected samples(including 112 samples of cervical cancer,151 samples of CIN Ⅰ,246 samples of CIN Ⅱ and 120 samples of CIN Ⅲ);the correlation between integrated state of HPV-16 and the severity of cervical lesions was analyzed. Results:Among the patients with cervical cancer,112 patients were infected by HPV-16,accounting for 56.57% of the total patients with cervical cancer;among 112 HPV-16 infected patients with cervical cancer,73 patients were found with simple integrated infection of HPV-16,the positive rate was 65.18%(73/112);the infection rates of simple integrated HPV-16 in the patients with CIN Ⅰ,CIN Ⅱ and CIN Ⅲ were 7.95%,23.58% and 39.17%,respectively;there was significant difference in the infection rate of simple integrated HPV-16 between the patients with cervical cancer and the patients with CIN Ⅰ,CIN Ⅱ and CIN Ⅲ. Conclusion:HPV-16 is the most common type of viral infection in patients with cervical cancer,among the patients with HPV-16 infection,the integrated state of HPV is positively correlated with the severity of cervical lesions.Assisted HPV typing test and detection of viral integrated state will be more helpful to predict the prognosis of patients with precancerous lesions of uterine cervix and increase the accuracy of cervical cancer screening on the basis of HC-II HPV DNA detection.