Screening for Bioactive Mutants with Antitumor Activity from HLF-43,a Marine-Derived Actinomycete Strain without Antitumor Activity,by Neomycin-Resistant Mutant Selection Coupled with Microwave Mutagenesis

Total 128 neomycin-resistant(neo) mutants including the 117 mutants from microwave mutagenesis and the 11 mutants from auto-mutagenesis were obtained by neo mutant selection on neomycin-containing plates coupled with microwave-induced mutation technique.Among the neo mutants obtained,the 8 neo mutants from microwave mutagenesis appeared to be bioactive with the inhibition rates over 20% at the 100μg/mL of sample concentration on K562 cells.By combination of the different time of microwave irradiation and the different concentration of neomycin,it has been appeared that the 120 s of microwave irradiation is likely to be favorable for obtaining more neo mutants.