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2012•Journal of International Obstetrics and GynecologyRequires access

Experimental Study of Mefordcare Phytic Acid Ketone Effect on HeLa Cells Using MTT Assay

Yue Tian-f

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Abstract

Objective:To study the ability of the No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone on inhibiting the proliferation of HeLa cells which were cultivated in vitro,and provide the evidence for futher experiment.Methods:The HeLa cells which were cultivated in vitro was treated by eight different concentrations for 24,48 and 72h,MTT assay was employed to examine the growth suppression of HeLa cells.Results:Contrast with the control group,the apoptosis of the cells treated with these two numbers of the drug was increased and the growth rate was decreased.And the inhibition of HeLa cells in partly is in a concentration-dependent and time-dependent manner.Conclusions:The No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone could significantly inhibit the proliferation of HeLa cells.

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Objective:To study the ability of the No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone on inhibiting the proliferation of HeLa cells which were cultivated in vitro,and provide the evidence for futher experiment.Methods:The HeLa cells which were cultivated in vitro was treated by eight different concentrations for 24,48 and 72h,MTT assay was employed to examine the growth suppression of HeLa cells.Results:Contrast with the control group,the apoptosis of the cells treated with these two numbers of the drug was increased and the growth rate was decreased.And the inhibition of HeLa cells in partly is in a concentration-dependent and time-dependent manner.Conclusions:The No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone could significantly inhibit the proliferation of HeLa cells.

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Available abstract

Objective:To study the ability of the No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone on inhibiting the proliferation of HeLa cells which were cultivated in vitro,and provide the evidence for futher experiment.Methods:The HeLa cells which were cultivated in vitro was treated by eight different concentrations for 24,48 and 72h,MTT assay was employed to examine the growth suppression of HeLa cells.Results:Contrast with the control group,the apoptosis of the cells treated with these two numbers of the drug was increased and the growth rate was decreased.And the inhibition of HeLa cells in partly is in a concentration-dependent and time-dependent manner.Conclusions:The No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone could significantly inhibit the proliferation of HeLa cells.

Key concepts: HeLa, MTT assay, Phytic acid, In vitro, Apoptosis, Ketone, Chemistry, Molecular biology

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