Genetic Diversity of Chinese Hu mulberry(Morus L.) Based on ISSR Analysis

[Objective]To investigate the genetic diversity of local varieties of Chinese Hu mulberry(Morus L.).[Method]The genetic diversity of 141 copies of Hu mulberry varieties was analyzed by ISSR molecular markers.A dendrogram of all 141 Hu mulberry varieties based on the genetic similarity coefficients using ISSR molecular markers was generated by UPGMA cluster method.[Result] 12 ISSR primers had amplified a tota1 of 90 amplified,of which 57 bands were polymorphic,and the polymorphic rate was 63.33%.The genetic similarity coefficients of 141 Hu mulberry germplasm resources varied from 0.633 3 to 1.000 0 with the average of 0.483 4,indicating that there was difference on genetic diversity among different varieties of Hu mulberries.Clustering of the 141 Hu mulberry varieties did not correspond with the conventional morphology and agronomy characteristics.[Conclusion]Four subgroups clearly represent the genetic relationships in the 141 accessions which are benefit for the variety improvement and germplasm resource conservation.