1999•PubMedRequires access

[cDNA cloning of a novel gene related to apoptosis by differential display RT-PCR].

G Wang, Li X, Y Chen

Abstract

OBJECTIVE: To search for the novel gene related to apoptosis. METHODS: The mRNA expression patterns of U937 cells treated with or without trichosanthin were compared with differential display RT-PCR. The cDNA, which was highly expressed in the apoptosis cells, was used as a probe to screen the human fetal liver cDNA library. RESULTS: A 1.4 kb cDNA segment was the partial sequence of a novel gene related to apoptosis. The result of Northern blot showed three bands in the apoptosis cells. Some sequence of the 1.4 kb cDNA was homology to Bruton's tyrosine kinase. CONCLUSION: A partial cDNA of a new gene related to apoptosis was found.

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Available abstract

Key concepts: Complementary DNA, Molecular biology, Differential display, cDNA library, Biology, Gene, Apoptosis, Northern blot