Isolation,identification and drug sensitivity of pathogen from goldfish

Eight dominant bacteria were isolated from the abodominal organs(liver,kidney and gonad)of the diseased goldfish using the conventional method.Among the eight strains,three bateria,temporarily named LK-T,PL-R and PK-T,were proved to be pathogenic,and were identified by ATB bacteria identification instrument.The results showed that strain LK-T and PL-R were Aeromonas sobria,strain PK-T was Aeromonas punctata subsp.caviae.The three pathogenic bacteria were all gram-negative and rod-shaped.They were highly sensitive to netilmicin,cefuroxime,cefradine,gentamicin,ciprofloxacin,neomycin and cefobid.Therefore,gentamicin,ciprofloxacin,and neomycin could be used for the control of this disease.