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2004•Chinese Journal of OsteoporosisRequires access

Effect of simvastatin on differentiation of osteoblasts derived from bone marrow stem cells.

Qiang Qu

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Abstract

Objective To study the effect of simvastatin on osteoblast differentiation by using a mouse bone marrow culture system.Methods Bone marrow cells were derived from 6-week-old ICR mice. Dexamethasone was initiated in the cultures. Alkaline phosphatase staining and von Kossa staining were used to assess osteoblast differentiation. Semi-quantitative RT-PCR was perfermod for evaluating the expressions of collagen type I (COL1), akaline phosphatase (ALP), CBFA1, and Osterix on culture days 3 and 5, in the simvastatin-(treated) gron and blank control group.Results Cells in each group were differentiated into osteoblast lineage, as shown by alkaline phosphatase and von Kossa stainings. The expressions of COL1, ALP and CBFA1 in the treatment group was higher than those in the control group.Conclusion Simvastatin enhances the expression of osteoblast-differentiation-related genes.

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What this paper is about

Objective To study the effect of simvastatin on osteoblast differentiation by using a mouse bone marrow culture system.Methods Bone marrow cells were derived from 6-week-old ICR mice. Dexamethasone was initiated in the cultures. Alkaline phosphatase staining and von Kossa staining were used to assess osteoblast differentiation. Semi-quantitative RT-PCR was perfermod for evaluating the expressions of collagen type I (COL1), akaline phosphatase (ALP), CBFA1, and Osterix on culture days 3 and 5, in the simvastatin-(treated) gron and blank control group.Results Cells in each group were differentiated into osteoblast lineage, as shown by alkaline phosphatase and von Kossa stainings. The expressions of COL1, ALP and CBFA1 in the treatment group was higher than those in the control group.Conclusion Simvastatin enhances the expression of osteoblast-differentiation-related genes.

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Available abstract

Objective To study the effect of simvastatin on osteoblast differentiation by using a mouse bone marrow culture system.Methods Bone marrow cells were derived from 6-week-old ICR mice. Dexamethasone was initiated in the cultures. Alkaline phosphatase staining and von Kossa staining were used to assess osteoblast differentiation. Semi-quantitative RT-PCR was perfermod for evaluating the expressions of collagen type I (COL1), akaline phosphatase (ALP), CBFA1, and Osterix on culture days 3 and 5, in the simvastatin-(treated) gron and blank control group.Results Cells in each group were differentiated into osteoblast lineage, as shown by alkaline phosphatase and von Kossa stainings. The expressions of COL1, ALP and CBFA1 in the treatment group was higher than those in the control group.Conclusion Simvastatin enhances the expression of osteoblast-differentiation-related genes.

Key concepts: Alkaline phosphatase, Osteoblast, Von Kossa stain, Simvastatin, Bone marrow, Staining, Dexamethasone, Chemistry

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Effect of simvastatin on differentiation of osteoblasts derived from bone marrow stem cells. — Research Paper | ScholarLens